Program Details:

9:30 - 10:00

Breakfast, Welcome

10:00 - 11:30

Modern Assays and Technologies in Fluorometry and Luminometry
Presented by: Marika Raitio, Application Scientist; Hanna Granö-Fabritius, Product Line Manager

Introduction to detection technologies

• Principles of the technologies
• How instruments operate
• Choosing the best technology for the assay

Reporter genes

• Green fluorescent proteins (GFPs) and analogues
• Luciferase reporter genes

Resonance Energy Transfer technology

• FRET, TR-FRET, and BRET
• Operational principles, benefits, and examples

Quantum Dot (Qdot) dye technology

• Structure of the dyes, benefits, and examples

Toxicity assays

• Fluorometric Multiparameter toxicity assay

Customer Case

11:30 - 11:45

Coffee Break

11:45 - 12:45

Result quality: Features of Microplate Readers to Facilitate High Quality Assays
Presented by: Marika Raitio, Application Scientist; Hanna Granö-Fabritius, Product Line Manager

General features of microplate assays affecting data quality

• Relative nature of the result data
• Vertical microplate photometry vs. cuvette photometry
• Microplates

Specific Issues: Fluorometry and Luminometry

• Long-term stability of the instruments
• Selection of the measurement gain
• PMT stability and gain control
• Lamp control
• Instrument background noise control
• Luminometric cross talk control

Specific Issues: Photometry

• Base level stabilization
• Flash intensity controls
• Path length correction - What is this and
  does it help?

Customer Case

12:45 - 13:30

Instrument and Software Demonstrations

13:30 - 14:30

Lunch